Validated miRNA GoClone Targets Experimental Data
Jump to: miR-124 | miR-1 | miR-122 | miR-29a | miR-21 | miR-208a | miR-200a | let-7a | miR-155
The putative 3’ UTR targets of miR-124 were initially identified by an Ago2-IP in the presence of miR-124 mimic (Hendrickson et al. Plos ONE 2008*). We co-transfected putative miR-124 targets along with either 50nM of miR-124 mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-1 were initially identified by an Ago2-IP in the presence of miR-1 mimic (Hendrickson et al. Plos ONE 2008*). We co-transfected putative miR-1 targets along with either 20nM of miR-1 mimic or a non-targeting control miRNA in HeLa cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-122 were initially predicted by the miRanda algorithm. We co-transfected putative miR-122 targets along with either 20nM of miR-122 mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-29a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-21 were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-208a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-200a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 20nM of miRNA mimic or a non-targeting control miRNA in HeLa cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of let7a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-155 were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
* References
1. Hendrickson DG, Hogan DJ, Herschlag D, Ferrell JE, Brown PO. Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance. PLoS One. 2008 May 7;3(5):e2126.