GoClone Validated miRNA Targets Experimental Data
Jump to: miR-124 | miR-223 | miR-122 | miR-29 | miR-21 | miR-208a | miR-200a | let-7a | miR-155
The putative 3’ UTR targets of miR-124 were initially identified by an Ago2-IP in the presence of miR-124 mimic (Hendrickson et al. Plos ONE 2008*). We co-transfected putative miR-124 targets along with either 50nM of miR-124 mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-223 were initially identified by a high-throughput mass spectrometry screen in the presence of miR-223 mimic (Baek et al. Nature 2008*). We co-transfected putative miR-223 targets along with either 50nM of miR-223 mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-122 were initially predicted by the miRanda algorithm. We co-transfected putative miR-122 targets along with either 20nM of miR-122 mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-29 were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-21 were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-208a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-200a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of let7a were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
The putative 3’ UTR targets of miR-155 were initially predicted by the TargetScan algorithm. We co-transfected putative miRNA targets along with either 50nM of miRNA mimic or a non-targeting control miRNA in K562 cells. We selected the four strongest responders from this experiment along with two non-responding controls.
* References
1. Hendrickson DG, Hogan DJ, Herschlag D, Ferrell JE, Brown PO. Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance. PLoS One. 2008 May 7;3(5):e2126.
2. Baek D, Villén J, Shin C, Camargo FD, Gygi SP, Bartel DP. The impact of microRNAs on protein output. Nature. 2008 Sep 4;455(7209):64-71.