Heat Shock (HSF) Pathway Products Experimental Data

Introduction

SwitchGear Genomics offers transfection-ready reporter constructs to allow you to measure Heat Shock pathway activation. The SwitchGear Heat Shock promoter targets were picked from our genome-wide promoter collection based on sequence motif analysis, expression studies, and transcription factor binding data. We conducted experiments to generate a profile across the set of constructs with and without Heat Shock activation and offer a set of key responder constructs that show a strong induction response that can be used as biomarkers in screening applications.

Heat Shock Biomarker Set – 8 promoters, 2 controls

The selected constructs shown in the graph are the 4 of the highest responding promoters under our chosen test conditions*. The chart summarizes the induction ratios for each construct in HepG2 human hepatocarcinoma cells that were transfected with promoter-reporter constructs and treated with 1uM lovastatin or vehicle only for 24 hours in reduced lipid media. The Y-axis shows the treated/untreated signal ratio on a log scale and error bars represent one standard deviation. View more information on building this set.