P53 Pathway Products
The SwitchGear p53 Pathway Screening Sets
P53, a transcription factor that regulates the cell cycle, is well-characterized for its role in tumor suppression. A broad range of stressors such as hypoxia, DNA damage, and viral infection stabilize and activate p53, inducing a large cascade of genes that play a significant role in cell cycle arrest, DNA repair, and apoptosis.
SwitchGear Genomics offers a comprehensive panel of transfection-ready, cell-based promoter reporter assays to allow you to accurately quantify transcriptional activation and repression in response to p53 pathway activation. In addition, SwitchGear offers UTR reporter assays that measure transcript stability, translational repression, miRNA function.
Applications
The SwitchGear collection of p53-relevant promoter reporters allows you to:
- Understand the mechanisms by which p53- regulated genes are induced or repressed
- Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor level, our promoter reporter assays quantify the effects of transcription factor binding
- Confirm data from ChIP-chip or ChIP-sequencing experiments
- Measure the effect of sequence variants and mutagenesis on promoter function
- Screen for promoter activation or develop activity profiles across the p53 signaling pathway for many compounds or conditions in parallel
SwitchGear also offers 3′UTR reporters for studying transcriptional stability, translational repression, and miRNA function. Read more about this technology.
Promoter Reporter Technology
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The Power of the LightSwitch Luciferase Assay System
- Quantitative: Novel RenSP luciferase fusion technology allows you to measure promoter activity in response to p53 pathway activation with industry-leading sensitivity and dynamic range
- Simple, quick, complete solution: Perform your experiments today through simple transfection of our luciferase reporter constructs using LightSwitch optimized reagents. No cloning, DNA preparation, or reagent optimization is needed. No need for co-transfection of normalizing controls.
- Comprehensive and verified: Choose from SwitchGear’s complete set of sequence verified and transfection ready p53 targets.
- Functionally insightful: Learn about the actual effects of transcription factor binding to verify computational predictions and supplement microarray or sequencing data.
- Cost-effective: Profile promoter activation efficiently across the full p53 pathway under a multitude of conditions in parallel.