The SwitchGear TGF-beta Pathway Panel

Transforming growth factor beta (TGF-beta) controls a large number of processes through its various signaling pathways, including cell cycle regulation, cellular proliferation, differentiation, apoptosis, and angiogenesis. Aberrant TGF-beta regulation plays a role in cardiovascular disease, diabetes, immune disorders, oncogenesis, and Marfan syndrome. TGF-beta is typically activated by ligand-receptor binding which leads to the activation of the SMAD and DAXX pathways, the control of SMAD protein activity and degradation, and the transcriptional regulation of target genes.

As constitutive activation of the TGF-beta/SMAD pathways can lead to tumor growth, irregular embryogenesis and various other diseases, modulating TGF-beta signaling may confer therapeutic benefit for many physiological disorders. SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of TGF-beta/SMAD promoter reporters and TGF-beta/SMAD UTR reporters to allow you to accurately quantify transcriptional regulation and translational efficiency in the TGF-beta pathways.

Products: TGF-beta/SMAD reporter assays

Click image for a larger view of the TGF-beta pathway.

SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.

SwitchGear offers genome-wide, assay-ready promoter and UTR reporters for studying transcript regulation and stability, including specific panels for TGF-beta/SMAD. Please note that custom pricing is available for orders of 10 or more constructs. Contact us for more information.

Our TGF-beta/SMAD Promoter Reporter Collection enables you to quantify promoter induction and more fully assess transcriptional activation driven by specific transcription factors.
Our TGF-beta/SMAD UTR Reporter Collection allows you to quantify 3′UTR impact on transcript stability and translational efficiency. Or search SwitchDB for specific miRNA candidates.

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.

Applications

The SwitchGear collection of TGF-beta-relevant promoter reporters allows you to:

Understand the mechanisms by which the TGF-beta signaling pathway of genes is induced or repressed
Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
Confirm data from ChIP-chip or ChIP-sequencing experiments
Measure the effect of sequence variants and mutagenesis on promoter function
Screen for promoter activation in the full TGF-beta signaling pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of TGF-beta-relevant 3′UTR reporters allows you to:

Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
Validate expression data and computational predictions with miRNA functional studies
Measure the effect of sequence variants and mutagenesis on 3′UTR function
Screen for UTR and miRNA function in the full TGF-beta pathway

See UTR Reporter Collection for general information. Or search SwitchDB for specific miRNA candidates.

The SwitchGear Advantage

Quantitative: Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.
Comprehensive and validated: Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.
Simple and quick: Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.