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The SwitchGear TGF-beta Pathway Panel

As constitutive activation of the TGF-beta/SMAD pathways can lead to tumor growth, irregular embryogenesis and various other diseases, modulating TGF-beta signaling may confer therapeutic benefit for many physiological disorders. SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of  TGF-beta/SMAD promoter reporters and TGF-beta/SMAD UTR reporters to allow you to accurately quantify transcriptional regulation and translational efficiency in the TGF-beta pathways.

 

Products: TGF-beta/SMAD reporter assays

Click image for a larger view of the TGF-beta pathway.
tgf-pathway-pic1
SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.
SwitchGear offers genome-wide, assay-ready promoter and UTR reporters for studying transcript regulation and stability, including specific panels for TGF-beta/SMAD.  Please note that custom pricing is available for orders of 10 or more constructs. Contact us for more information.

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.

 

 

Applications

The SwitchGear collection of TGF-beta-relevant promoter reporters allows you to:

  • Understand the mechanisms by which the TGF-beta signaling pathway of genes is induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation in the full TGF-beta signaling pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of TGF-beta-relevant 3′UTR reporters allows you to:

  • Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
  • Validate expression data and computational predictions with miRNA functional studies
  • Measure the effect of sequence variants and mutagenesis on 3′UTR function
  • Screen for UTR and miRNA function in the full TGF-beta pathway

See UTR Reporter Collection for general information. Or search SwitchDB for specific miRNA candidates.

 

 

 

The SwitchGear Advantage

  • Quantitative: Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.
  • Comprehensive and validated: Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.
  • Simple and quick: Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.