The NFAT Reporter Collection

Nuclear factor of activated T-cells (NFAT) refers to the family of transcription factors involved in regulation of immune response and development of the skeletal, cardiac, and nervous systems. Misregulation of the NFAT pathways has also been implicated in Down’s syndrome. Understanding the mechanisms of gene regulation in the NFAT signaling pathways provides key insights in NFAT signaling-associated disease and is important for potential therapeutic development.
SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of NFAT-relevant promoter reporters and NFAT-relevant UTR reporters allowing you to accurately quantify transcriptional regulation, transcript stability, and translational efficiency of genes in the NFAT signaling pathway. Uncover miRNA function and the effects of transcription binding in the full NFAT pathway of genes.

 

NFAT Pathway Products

NFAT Canonical Pathway diagram | Click on the image for a larger view.
nfat-pathway-pic
SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.
SwitchGear offers promoter and UTR reporters for studying transcript regulation and stability. Custom pricing is available for orders of 10 or more constructs. Contact us! 

  • Our NFAT Promoter Reporter Collection enables you to quantify promoter induction and assess transcriptional activation driven by specific transcription factors.
  • Our NFAT UTR Reporter Collection allows you to quantify the 3′UTR impact on transcript stability under specific conditions (e.g. in the presence of an miRNA). Or search SwitchDB for specific miRNA candidates.

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.
 

Applications

The SwitchGear collection of NFAT-relevant promoter reporters allows you to:

  • Understand the mechanisms by which the NFAT pathway of genes is induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation in the full NFAT pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of NFAT-relevant 3′UTR reporters allows you to:

  • Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
  • Validate expression data and computational predictions with miRNA functional studies
  • Measure the effect of sequence variants and mutagenesis on 3′UTR function
  • Screen for UTR and miRNA function in the full NFAT pathway

See UTR Reporter Collection for general information. Or search SwitchDB for specific miRNA candidates.

 

The SwitchGear Advantage

QUANTITATIVE
Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.

COMPREHENSIVE AND VALIDATED
Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.

SIMPLE AND QUICK
Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.