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The Notch Signaling Pathway Reporter Collection

The Notch signaling pathway plays a key role various cellular processes particularly in the regulation of cell-to-cell communication in embryonic development and differentiation, cell fate, and angiogenesis. In canonical Notch signaling, ligands such as Jagged and Delta trigger Notchs to regulate the activation of various gene cascades. Abnormal regulation of the Notch pathway has been implicated in brain cancer, prion disease, CADASIL, Alagille syndrome, and spondylocostal dysostosis.

As constitutive activation of the Notch pathway can lead to various disorders, understanding the mechanisms of Notch gene regulation provides important biological insights. SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of Notch promoter reporters and Notch UTR reporters to allow you to accurately quantify transcriptional regulation and translational efficiency in the Notch pathway.

 

Notch Pathway Products

Notch Canonical Pathway diagram | Click on the image for a larger view.
notch-pathway-pic1
SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.
SwitchGear offers promoter and UTR reporters for studying transcript regulation and stability. Custom pricing is available for 10 or more constructs. Contact us!

  • Our Notch Promoter Reporter Collection enables you to quantify promoter induction and more fully assess transcriptional activation driven by specific transcription factors.
  • Our Notch UTR Reporter Collection allows you to quantify the impact that a 3′UTR has on transcript stability under specific conditions (e.g. in the presence of an miRNA).

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.
 

Applications

The SwitchGear collection of Notch-relevant promoter reporters allows you to:

  • Understand the mechanisms by which the Notch pathway of genes is induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation in the full Notch pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of Notch-relevant 3′UTR reporters allows you to:

  • Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
  • Validate expression data and computational predictions with miRNA functional studies
  • Measure the effect of sequence variants and mutagenesis on 3′UTR function
  • Screen for UTR and miRNA function in the full Notch pathway

See UTR Reporter Collection for general information.

 

The SwitchGear Advantage

QUANTITATIVE
Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.

COMPREHENSIVE AND VALIDATED
Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.

SIMPLE AND QUICK
Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.