The Oct4 Signaling Pathway Reporter Collection

The Oct4 signaling pathway plays a critical role in embryonic development and differentiation, specifically as Oct4 has been identified as a critical master regulator in maintaining embryonic stem cell pluripotency. Aberrant expression of Oct4 leads to various abnormalities in various embryonic stages and has also been linked to tumorigenesis in adult germ cells. Understanding the mechanisms of Oct4 signaling is a key piece in understanding embryogenesis, development, and potentially disease progression and prevention.

SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of Oct4 promoter reporters and Oct 4 UTR reporter assays to allow you to accurately quantify transcriptional regulation, transcript stability, translational efficiency in the Oct4 signaling pathway.

  

Oct4 Pathway Products

Oct4 Canonical Pathway diagram | Click on the image for a larger view.
oct4-pathway-pic1
SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.
SwitchGear offers promoter and UTR reporters for studying transcript regulation, transcript stability, and translational efficiency. Custom pricing is available for orders of 10 or more constructs. Contact us!

  • Our Oct4 Promoter Reporter Collection enables you to quantify promoter induction and more fully assess transcriptional activation driven by specific transcription factors.
  • Our Oct4 UTR Reporter Collection allows you to quantify the impact that a 3′UTR has on transcript stability under specific conditions (e.g. in the presence of an miRNA).

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.
 

Applications

The SwitchGear collection of Oct4-relevant promoter reporters allows you to:

  • Understand the mechanisms by which the Oct4 signaling pathway of genes is induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation in the full Oct4 pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of Oct4-relevant 3′UTR reporters allows you to:

  • Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
  • Validate expression data and computational predictions with miRNA functional studies
  • Measure the effect of sequence variants and mutagenesis on 3′UTR function
  • Screen for UTR and miRNA function in the full Oct4 signaling pathway

See UTR Reporter Collection for general information.

  

The SwitchGear Advantage

QUANTITATIVE
Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.

COMPREHENSIVE AND VALIDATED
Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.

SIMPLE AND QUICK
Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.