The Sonic Hedgehog Signaling Pathway Reporter Collection

The Hedgehog signaling pathway is critical for growth and development. Sonic hedgehog (SHH) is particularly crucial in regulating vertebrate development and patterning, and defects of SHH have been implicated in numerous disorders including holoprosencephaly, immune disorders, and pulmonary disease.

SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of SHH promoter reporters and SHH UTR reporters to allow you to accurately quantify transcriptional regulation and translational efficiency in the hedgehog signaling pathway.

 

Products: SHH reporter assays

Click image for a larger view of the SHH canonical pathway.
shh-pathway-pic
SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.
SwitchGear offers genome-wide, assay-ready promoter and UTR reporters for studying transcript regulation and stability, including specific panels for SHH. Custom pricing available for 10 or more constructs! Contact Us!

  • Our SHH Promoter Reporter Collection enables you to quantify promoter induction and more fully assess transcriptional activation driven by specific transcription factors.
  • Our SHH UTR Reporter Collection allows you to quantify 3′UTR impact on transcript stability and translational efficiency and study miRNA function. Or search SwitchDB for specific miRNA candidates.

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.

 

Applications

The SwitchGear collection of SHH-relevant promoter reporters allows you to:

  • Understand the mechanisms by which the SHH signaling pathway of genes is induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation in the full SHH signaling pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of SHH-relevant 3′UTR reporters allows you to:

  • Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
  • Validate expression data and computational predictions with miRNA functional studies
  • Measure the effect of sequence variants and mutagenesis on 3′UTR function
  • Screen for UTR and miRNA function in the full SHH pathway

See UTR Reporter Collection for general information. Or search SwitchDB for specific miRNA candidates.

 

The SwitchGear Advantage

QUANTITATIVE
Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.

COMPREHENSIVE AND VALIDATED
Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.

SIMPLE AND QUICK
Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.