The SwitchGear Wnt Pathway Panel

The richness of Wnt signaling illustrates its significance as a key regulator of the expression of a multitude of physiologically important genes. As constitutive activation of the Wnt pathway can lead to tumorigenesis and improper stem cell self-renewal, modulation of Wnt signaling may confer therapeutic benefit for both tissue regeneration in numerous diseases and inhibition of cancer cells. SwitchGear Genomics offers genome-wide panels of transfection-ready, cell-based reporter assays including specific panels of  Wnt promoter reporters and Wnt UTR reporter assays to allow you to accurately quantify transcriptional regulation and translational efficiency in the Wnt pathways.

 

Wnt Pathway Products

Wnt Canonical Pathway diagram
Click on the image for a larger view.
wnt-pathway-pic
SwitchGear Genomics acknowledges Ingenuity Systems®, Inc. www.ingenuity.com for the biological pathway diagram and supporting content.
SwitchGear offers promoter and UTR reporters for studying transcript regulation and stability. Custom pricing is available for orders of 10 or more constructs. Contact us! 

  • Our Wnt Promoter Reporter Collection enables you to quantify promoter induction and more fully assess transcriptional activation driven by specific transcription factors.
  • Our Wnt UTR Reporter Collection allows you to quantify the impact that a 3′UTR has on transcript stability under specific conditions. Or search SwitchDB for specific miRNA candidates.

View our genome-wide collection of promoter and UTR products in our online catalog, SwitchDB.

 

 

Applications

The SwitchGear collection of Wnt-relevant promoter reporters allows you to:

  • Understand the mechanisms by which the Wnt pathway of genes is induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor presence, our promoter reporter assays quantify the effects of transcription factor binding.
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation in the full Wnt pathway under a multitude of conditions

See Promoter Reporter Collection for general information.

The SwitchGear collection of Wnt-relevant 3′UTR reporters allows you to:

  • Quantify the functional role of UTRs and miRNAs in post-transcriptional gene regulation under a multitude of conditions
  • Validate expression data and computational predictions with miRNA functional studies
  • Measure the effect of sequence variants and mutagenesis on 3′UTR function
  • Screen for UTR and miRNA function in the full Wnt pathway

See UTR Reporter Collection for general information. Or search SwitchDB for specific miRNA candidates.

 

  

 

The SwitchGear Advantage

  • Quantitative: Quantify miRNA impact or consequences of transcription factor binding through highly quantitative luciferase activity.
  • Comprehensive and validated: Choose from Switchgear’s catalog of 7,000 sequence-verified UTR constructs and 10,000 promoter constructs.
  • Simple and quick: Perform your experiments today through a simple transfection of our UTR or promoter constructs. No cloning or DNA preparation required.

Wnt Application Highlight

Two recent studies describe newly discovered, specific roles for the Wnt signaling cascade in cancer. A group at Memorial Sloan-Kettering Cancer Center (MSKCC) recently demonstrated the role of the Wnt/Tch pathway in lung cancer metastasis. Investigation of six different pathways revealed that the Wnt pathway was the only one with hyperactive levels in metastasized tumors yet normal levels in non-metastasized tumors. The researchers identified two genes activated by Wnt –HOXB9 and LEF1– that enhance the ability of lung cancer cells to metastasize.1

In another recent study, Stanford researchers discovered that the TERT protein (a component of the telomerase complex) directly regulates the Wnt pathway by occupying the upstream promoter regions of Wnt genes. Various transcription factors have been shown to regulate the Wnt genes including TCF-4 and GATA2, so the discovery of TERT regulation was novel. The study also revealed that TERT overexpression in the presence of a remodeling protein induced Wnt genes. Finally, the study showed that TERT is essential for mouse stem cell response to Wnt signals. The researchers believe that the data indicates that blocking Wnt-associated activity may be more efficient then blocking telomerase for inhibiting cancer cells. In short, the Wnt pathway and telomerase activity show closely related functions in stem cell self-renewal and cancer cell proliferation.