pLightSwitch_5UTR

Base pairs                                              4624
human ACTB promoter and 5'UTR							35-1080
Multiple cloning region                                 1014-1080
RenSP reporter gene (synthetic renilla luciferase;
 includes mODC PEST)                                    1080-2138
SV40 late poly(A) region                                2169-2390 
ColE1-derived plasmid replication origin                2715
Synthetic Beta-lactamase (Ampr) coding region           3506-4366
Synthetic poly(A) signal/transcriptional pause site     4471-4624

GGCCTAACTGGCCGGTACCTGAGCTCTTACGCGTATAGCAGACATACAACGGACGGTGGG
CCCAGACCCAGGCTGTGTAGACCCAGCCCCCCCGCCCCGCAGTGCCTAGGTCACCCACTA
ACGCCCCAGGCCTTGTCTTGGCTGGGCGTGACTGTTACCCTCAAAAGCAGGCAGCTCCAG
GGTAAAAGGTGCCCTGCCCTGTAGAGCCCACCTTCCTTCCCAGGGCTGCGGCTGGGTAGG
TTTGTAGCCTTCATCACGGGCCACCTCCAGCCACTGGACCGCTGGCCCCTGCCCTGTCCT
GGGGAGTGTGGTCCTGCGACTTCTAAGTGGCCGCAAGCCACCTGACTCCCCCAACACCAC
ACTCTACCTCTCAAGCCCAGGTCTCTCCCTAGTGACCCACCCAGCACATTTAGCTAGCTG
AGCCCCACAGCCAGAGGTCCTCAGGCCCTGCTTTCAGGGCAGTTGCTCTGAAGTCGGCAA
GGGGGAGTGACTGCCTGGCCACTCCATGCCCTCCAAGAGCTCCTTCTGCAGGAGCGTACA
GAACCCAGGGCCCTGGCACCCGTGCAGACCCTGGCCCACCCCACCTGGGCGCTCAGTGCC
CAAGAGATGTCCACACCTAGGATGTCCCGCGGTGGGTGGGGGGCCCGAGAGACGGGCAGG
CCGGGGGCAGGCCTGGCCATGCGGGGCCGAACCGGGCACTGCCCAGCGTggggcgcgggg
gccacggcgcgcgcccccagcccccgggcccAGCACCCCAAGGCGGCCAACGCCAAAACT
CTCCCTCCTCCTCTTCCTCAATCTCGCTCTCGCTCTTTTTTTTTTTCGCAAAAGGAGGGG
AGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAAGCCGGTGAGTGAGCGGCGCGGGGCC
AATCAGCGTGCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGCGGCCGCGGCGGCGCCC
TATAAAACCCAGCGGCGCGACGCGCCACCACCGCCGAGACCGCGTCCGCCCCGAGATCTC
AGAGCCTCGCCTTTCTTAAGCCGATCCGCCGGACGTCCACACCCCCTGCAGGCTCACCCA
TGGCTTCCAAGGTGTACGACCCGGAGCAGCGCAAGAGGATGATCACCGGCCCTCAGTGGT
GGGCTCGGTGCAAGCAGATGAACGTGCTCGACTCCTTCATCAACTACTACGACAGCGAGA
AACATGCGGAGAACGCCGTGATCTTCCTCCACGGCAACGCCGCTTCCTCCTACCTGTGGC
GCCACGTCGTGCCCCACATCGAGCCCGTCGCCCGGTGCATCATCCCTGATCTGATCGGGA
TGGGGAAGAGCGGGAAGAGCGGCAACGGCAGCTACCGCCTGCTCGACCACTACAAGTACC
TCACCGCCTGGTTCGAGCTGCTGAACCTCCCCAAGAAGATCATCTTTGTGGGCCACGACT
GGGGCGCTTGTCTCGCTTTTCACTACTCCTACGAGCACCAGGATAAGATCAAGGCTATCG
TGCATGCTGAGAGCGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCCGATATCGAGG
AGGATATTGCTCTGATCAAGTCCGAGGAGGGCGAGAAGATGGTCCTGGAGAATAACTTCT
TCGTGGAGACTATGCTGCCTAGCAAGATCATGCGCAAGCTGGAGCCCGAGGAGTTCGCTG
CTTACCTGGAGCCCTTCAAGGAGAAGGGCGAGGTCAGAAGACCAACCCTCAGCTGGCCTC
GGGAGATCCCTCTGGTCAAGGGCGGGAAGCCGGACGTGGTGCAGATCGTCCGGAACTACA
ACGCCTACCTGCGCGCCAGCGACGACCTGCCTAAGATGTTCATCGAGTCCGACCCCGGCT
TCTTCAGCAACGCTATCGTGGAGGGCGCCAAGAAGTTCCCCAACACCGAGTTCGTGAAGG
TGAAGGGCCTCCACTTCTCCCAAGAGGACGCCCCTGATGAGATGGGGAAGTACATCAAGA
GCTTCGTCGAGCGCGTCCTCAAGAACGAGCAGAATTCTCACGGCTTCCCTCCCGAGGTGG
AGGAGCAGGCCGCCGGCACCCTGCCCATGAGCTGCGCCCAGGAGAGCGGCATGGATAGAC
ACCCTGCTGCTTGCGCCAGCGCCAGGATCAACGTCTAATCTAGAGTCGGGGCGGCCGGCC
GCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCA
GTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTAT
AAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGG
GGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAA
GGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGC
GGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGAC
AGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGG
GATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAG
GCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA
CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT
GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCC
TTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCG
GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC
TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA
CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG
TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCT
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACC
ACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGA
TCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA
CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT
TAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCGGC
CGCAAATGCTAAACCACTGCAGTGGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAG
CGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGA
TACGGGAGGGCTTACCATCTGGCCCCAGCGCTGCGATGATACCGCGAGAACCACGCTCAC
CGGCTCCGGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTC
CTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTA
GTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATCGCTACAGGCATCGTGGTGTCAC
GCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT
GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAA
GTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTG
TCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAG
AATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGC
CACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGAT
CTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATG
CCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCGTCCTTTTTC
AATATTATTGAAGCATTTATCAGGGTTACTAGTACGTCTCTCAAGGATAAGTAAGTAATA
TTAAGGTACGGGAGGTATTGGACAGGCCGCAATAAAATATCTTTATTTTCATTACATCTG
TGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAACAAAACG
AAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTT
CTCT