CREB Pathway Products

SwitchGear CREB Pathway Screening Sets

Measure the activity of regulatory elements across the full CREB/cAMP-related pathway with transfection-ready promoter and 3′UTR assays

The CREB (cAMP responsive element binding protein) signaling pathway regulates a variety of biological processes including cell survival and proliferation, glucose regulation, neuronal differentiation, and long-term memory formation. The CREB transcription factor family — CREB, CRE-modulatory protein (CREM) and activating transcription factor 1 (ATF1) activate transcription by binding to CREs in the promoter regions of target genes. CREB responds to a number of stimuli including various important signal transduction cascades. The richness of CREB signaling illustrates its significance as a key regulator of the expression of a multitude of physiologically important genes.

SwitchGear Genomics offers a comprehensive panel of transfection-ready, cell-based promoter reporter assays to allow you to accurately quantify transcriptional activation and repression in response to CREB pathway activation. In addition, SwitchGear offers UTR reporter assays that measure transcript stability, translational repression, miRNA function.


Applications

The SwitchGear collection of CREB-related promoter reporters allows you to:

  • Understand the mechanisms by which CREB regulated genes are induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor level, our promoter reporter assays quantify the effects of transcription factor binding
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation or develop activity profiles across the CREB signaling pathway for many compounds or conditions in parallel

SwitchGear also offers 3′UTR reporters for studying transcriptional stability, translational repression, and miRNA function. Read more about this technology.


Promoter Reporter Technology
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The Power of the LightSwitch Luciferase Assay System

  • Quantitative: Novel RenSP luciferase fusion technology allows you to measure promoter activity in response to CREB pathway activation with industry-leading sensitivity and dynamic range
  • Simple, quick, complete solution: Perform your experiments today through simple transfection of our luciferase reporter constructs using LightSwitch optimized reagents. No cloning, DNA preparation, or reagent optimization is needed. No need for co-tranfection of normalizing controls.
  • Comprehensive and verified: Choose from SwitchGear’s complete set of sequence verified and transfection ready CREB targets.
  • Functionally insightful: Learn about the actual effects of transcription factor binding to verify computational predictions and supplement microarray or sequencing data.
  • Cost-effective: Profile promoter activation efficiently across the full CREB pathway under a multitude of conditions in parallel.

Additional links

Getting started with SwitchGear promoter constructs