CREB Pathway Products
Introduction
SwitchGear Genomics offers a panel of 88 transfection-ready reporter constructs to allow you to measure CREB pathway activation. The SwitchGear CREB promoter targets were picked from our genome-wide promoter collection based on sequence motif analysis, expression studies, and transcription factor binding data. We conducted experiments to generate a profile across the set of constructs with and without CREB activation. These data are summarized in the heatmap below. SwitchGear offers the complete pathway profiling panel of constructs in a plate format that allows you to characterize the effects of many conditions across the entire CREB pathway. We also offer a smaller subset of key responder constructs that show a strong induction response and can be used as biomarkers in screening applications.
CREB Biomarker Set - 8 promoters, 2 controls
The selected constructs shown in the graph are 8 of the highest responding promoters under our chosen test conditions*. The chart summarizes the induction ratios for each construct in Hela cells that were transfected with promoter-reporter constructs and treated with 100nM PKA or vehicle only for 4 hours. The Y-axis shows the treated/untreated signal ratio on a log scale and error bars represent one standard deviation. Please see the heatmap below for relative promoter activities in an untreated setting.
CREB Profiling Plate - 88 promoters, 8 controls
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The SwitchGear Profiling Plate is summarized in the heatmap in which each row represents a single promoter. The left side of the heatmap summarizes the untreated activity of each promoter where the intensity of yellow is proportional to the activity in an untreated condition. The right side of the heatmap summarizes the log2 ratios of treated/untreated activity of each promoter. Black indicates no change, and the intensity of red or blue indicates levels of induction or repression, respectively, in the conditions indicated at the top of the heatmap. (“PMA”= Hela cells treated with 100nM PMA for 4 hours; “FSK”= Hela cells treated with 40uM forskolin for 4 hours, 40 uM forskolin).
*Experimental results and absolute values of luciferase units may vary depending on cell line, treatment, and protocols. The data should be used as a guide for selecting candidate promoters and panels for your particular experimental design.