pLightSwitch_3UTR Base pairs 3910 RPL10 Promoter 33-307 RenSP reporter gene (synthetic renilla luciferase; includes mODC PEST) 350-1408 Multiple cloning region 2 (UTR region) 1409-1446 SV40 late poly(A) region 1455-1676 ColE1-derived plasmid replication origin 2001 Synthetic Beta-lactamase (Ampr) coding region 2792-3652 Synthetic poly(A) signal/transcriptional pause site 3757-3910 GGCCTAACTGGCCGGTACCTGAGCTCACGCGTGTACCCGGTCACCTCTCTGATCTGCGCA TGTGCTGGGCTACGCGCGGGCGCAAGCGCCAAGAGCGGCTGCGTCTATGGTCATGACGTC TGACAGAGCGTCCACCCGTCTTCGACAGGACTCTATGGTTCTTACGCGCGCAGACAGACC GCCTATATAAGCCATGCGCAGGCGGAGGAGCGCCTCTTTCCCTTCGGTGTGGGGAGCAAG CGCAGTTGTCGTCTCTTGCGGTGCCGTCGCTGGTTCTCACACCTTTTAGGTCTGTTCTCG TCTTCCCGAGATCTAAGCTTGGCATTCCGGTACTGTTGGTAAAGCCACCATGGCTTCCAA GGTGTACGACCCGGAGCAGCGCAAGAGGATGATCACCGGCCCTCAGTGGTGGGCTCGGTG CAAGCAGATGAACGTGCTCGACTCCTTCATCAACTACTACGACAGCGAGAAACATGCGGA GAACGCCGTGATCTTCCTCCACGGCAACGCCGCTTCCTCCTACCTGTGGCGCCACGTCGT GCCCCACATCGAGCCCGTCGCCCGGTGCATCATCCCTGATCTGATCGGGATGGGGAAGAG CGGGAAGAGCGGCAACGGCAGCTACCGCCTGCTCGACCACTACAAGTACCTCACCGCCTG GTTCGAGCTGCTGAACCTCCCCAAGAAGATCATCTTTGTGGGCCACGACTGGGGCGCTTG TCTCGCTTTTCACTACTCCTACGAGCACCAGGATAAGATCAAGGCTATCGTGCATGCTGA GAGCGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCCGATATCGAGGAGGATATTGC TCTGATCAAGTCCGAGGAGGGCGAGAAGATGGTCCTGGAGAATAACTTCTTCGTGGAGAC TATGCTGCCTAGCAAGATCATGCGCAAGCTGGAGCCCGAGGAGTTCGCTGCTTACCTGGA GCCCTTCAAGGAGAAGGGCGAGGTCAGAAGACCAACCCTCAGCTGGCCTCGGGAGATCCC TCTGGTCAAGGGCGGGAAGCCGGACGTGGTGCAGATCGTCCGGAACTACAACGCCTACCT GCGCGCCAGCGACGACCTGCCTAAGATGTTCATCGAGTCCGACCCCGGCTTCTTCAGCAA CGCTATCGTGGAGGGCGCCAAGAAGTTCCCCAACACCGAGTTCGTGAAGGTGAAGGGCCT CCACTTCTCCCAAGAGGACGCCCCTGATGAGATGGGGAAGTACATCAAGAGCTTCGTCGA GCGCGTCCTCAAGAACGAGCAGAATTCTCACGGCTTCCCTCCCGAGGTGGAGGAGCAGGC CGCCGGCACCCTGCCCATGAGCTGCGCCCAGGAGAGCGGCATGGATAGACACCCTGCTGC TTGCGCCAGCGCCAGGATCAACGTCTAATCTAGAGCTAGCCCTAGGGATATCCTCGAGGG CCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAAC CATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGT TCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAAT CGATAAGGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGT GGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCG TAGGACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTC GTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGT AAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA AATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT CCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTG TCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCC GACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTA TCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATC TGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAA CAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAA AAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAA AACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTT TTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGAC AGCGGCCGCAAATGCTAAACCACTGCAGTGGTTACCAATGCTTAATCAGTGAGGCACCTA TCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAA CTACGATACGGGAGGGCTTACCATCTGGCCCCAGCGCTGCGATGATACCGCGAGAACCAC GCTCACCGGCTCCGGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAA GTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAG TAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATCGCTACAGGCATCGTGG TGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAG TTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTG TCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTC TTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCAT TCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATA CCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAA AACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCA ACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGC AAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCGTCC TTTTTCAATATTATTGAAGCATTTATCAGGGTTACTAGTACGTCTCTCAAGGATAAGTAA GTAATATTAAGGTACGGGAGGTATTGGACAGGCCGCAATAAAATATCTTTATTTTCATTA CATCTGTGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAAC AAAACGAAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGA ACATTTCTCT