MISSION® 3’UTR Lenti GoClones

Validate miRNA targets and study post-transcriptional gene regulation with lentiviral 3′UTR reporters

SwitchGear Genomics has partnered with Sigma-Aldrich to offer a genome-wide collection of MISSION® 3′UTR Lenti GoClones®. The new system utilizes viral vectors to deliver human 3′UTRs fused to SwitchGear’s novel RenSP reporter gene and optimized reagents. LightSwitch Luciferase Assay Reagents have been designed specifically for use with RenSP and deliver maximum sensitivity, dynamic range, and convenience.

Visit Sigma-Aldrich to purchase your MISSION® 3′UTR Lenti GoClones

1. Transduce a MISSION 3′UTR Lenti GoClone into your cell line
2. A constitutive promoter drives production of a hybrid LUC-3′UTR construct
3. Total luciferase output depends on the effect of the human 3′UTR on the hybrid’s stability and/or translational efficiency
4. Include a synthetic miRNA or miRNA inhibitor to measure its impact on transcript stability and/or translational efficiency

Benefits of using the MISSION® 3′UTR Lenti GoClone Products

  • Flexibility: Achieve reporter expression in a wide range of mammalian cell lines including differentiated and non-dividing cells
  • Experimental consistency: easily create stably expressing cell lines
  • Convenience: Sequence-verified, pre-cloned luciferase reporter constructs for over 10,000 human 3′UTRs
  • Optimized: All constructs use SwitchGear’s novel RenSP luciferase gene and optimized LightSwitch™ Luciferase Assay Reagents