Validated Pathway Assays
Experimentally-validated promoter reporters
These sets of promoter targets selected from our genome-wide LightSwitch™ Promoter Reporter GoClone® Collection have been experimentally validated with pathway-specific inductions to create pathway activity profiles. These promoter reporters have been selected based on motif analysis, published information, and significant pathway induction response based on our in-house data.
Transcription Factor | Pathway | Induction treatment |
HIF1a | Hypoxia | 1% oxygen, DFO |
NFkB | Inflammation | TNFa |
CREB | cyclic-AMP | forskolin, PMA |
HSF1 | Heat shock | 43 degrees |
p53 | DNA damage, apoptosis | nutlin |
STAT | Interferon | interferon alpha, interferon gamma |
SREBP | Cholesterol biosynthesis | statins, cholesterol |
SRF | Serum response | 20% FBS |
ER | Estrogen | beta-estradiol |
AR | Androgen | methyltrienolone (R1881) |
GR | Glucocorticoid | dexamethasone, prednisone, cortisone |