The LightSwitch™ 3´UTR Reporter GoClone® Collection

Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone^® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity.

SwitchGear offers a number of control constructs to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that SwitchGear’s unique RenSP luciferase technology eliminates the need for co-transfection of a normalizing control in most cases.

Do you need positive controls for miRNA-3′UTR interactions? Please see our Synthetic miRNA Target GoClones.

Ordering

3′UTRs of housekeeping genes Details
Product #	Name	Description	Coordinates	Ordering
S804753	ACTB_3UTR	3′UTR CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
S804821	LDHA_3UTR	3′UTR CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
S810112	PPIA_3UTR	3′UTR CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
S801378	GAPDH_3UTR	3′UTR CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
Random genomic fragments in 3′UTR vector Details
Product #	Name	Description	Coordinates	Ordering
S890001	R01_3UTR	RAND CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
S890002	R02_3UTR	RAND CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
S890003	R03_3UTR	RAND CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
S890004	R04_3UTR	RAND CONTROL	Get info	5ug ($250) 5ug ($250) 10ug ($450)
Empty 3′UTR vector
Product #	Name	Description	Coordinates	Ordering
S890005	EMPTY_3UTR	EMPTY VECTOR	Get info	FREE*	5ug

* One free empty vector construct (5ug) with any purchase

If you are interested in ordering more than 10ug of one or more control constructs, please contact our Sales Department for a custom quote.

Details

Empty 3′UTR vector: The empty 3′UTR vector contains only the luciferase gene (RenSP) and its constitutive promoter. This construct may serve as a positive control for the transfection because of its promoter. It may also be utilized for creating additional constructs.

Housekeeping gene 3′UTR vectors: Housekeeping control constructs contain the 3′UTRs for common housekeeping genes cloned downstream of the RenSP luciferase reporter.

Random 3′UTR control vectors: Random control constructs contain non-conserved, non-genic, and non-repetitive human genomic fragments.

	Figure 1. Example data for SwitchGear 3′UTR control vectors. Here 50ng of each vector was transfected with FuGene6 (Roche) into HT1080 cells. Absolute and relative activity will vary depending on cell line, experimental conditions, and transfection reagents.
Values shown in log scale. Relative activities will differ depending on cell type and conditions.