Protocols and Tech Notes

PROTOCOLS

GoClone Reporter Constructs
Transfection Protocol for Adherent Cells
Transfection Protocol for K562 Suspension Cells
Transfection Protocol for 3’UTR Constructs and miRNA Mimic | Watch the video »
High-throughput Transfection Protocol

Reagents & Kits
LightSwitch Assay Reagent Protocol (LS010, 100 assays)
LightSwitch Assay Reagent Protocol (LS100, 1000 assays)
FuGENE HD Product Insert
Transfection Optimization Kit Protocol
 

HELP GUIDES

LightSwitch Luciferase Assay System Manual
Getting Started with Your Promoter Assays
Getting Started with Your 3’UTR Assays
3’UTR Assays Normalization Tutorial
 

TECHNICAL NOTES

Do I Need a Co-transfection Control?
High-throughput microRNA Target Screening: miR-122 Case Study (tech note)
High-throughput microRNA Target Screening: miR-122 Case Study (poster)
Using High-throughput Promoter Assays to Examine Regulatory Pathways
SwitchGear Methods for Gene Model Construction and Transcription Start Site Prediction