Validated Pathway Assays

Experimentally-validated promoter reporters

These sets of promoter targets from our genome-wide promoter reporter collection have been experimentally validated with pathway-specific inductions to create pathway activity profiles. These promoter reporters have been selected based on motif analysis, published information, and significant pathway induction response based on our in-house data.

Transcription Factor Pathway Induction treatment
HIF1a Hypoxia 1% oxygen, DFO
NFkB Inflammation TNFa
CREB cyclic-AMP forskolin, PMA
HSF1 Heat shock 43 degrees
p53 DNA damage, apoptosis nutlin
STAT Interferon interferon alpha, interferon gamma
SREBP Cholesterol biosynthesis statins, cholesterol
SRF Serum response 20% FBS
ER Estrogen beta-estradiol
AR Androgen methyltrienolone (R1881)
GR Glucocorticoid dexamethasone, prednisone, cortisone