The LightSwitch Promoter Reporter GoClone Collection

Our comprehensive collection contains more than 18,000 endogenous human promoter reporter GoClones that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity.

SwitchGear offers a number of control constructs to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that SwitchGear’s unique RenSP luciferase technology eliminates the need for co-transfection of a normalizing control in most cases.

Ordering

Promoters of housekeeping genes (Positive controls) Details
Product # Name Description Coordinates Ordering  
S708908 RPL10_PROM PROM CONTROL Get info 5ug ($230)
Add to Cart
S717678 ACTB_PROM PROM CONTROL Get info 5ug ($230)
Add to Cart
S721624 GAPDH_PROM PROM CONTROL Get info 5ug ($230)
Add to Cart
S721613 LDHA_PROM PROM CONTROL Get info 5ug ($230)
Add to Cart
Random genomic fragments in promoter vector (Negative/Background controls) Details
Product # Name Description Coordinates Ordering  
S790001 R01_PROM RAND CONTROL Get info 5ug ($230)
Add to Cart
S790002 R02_PROM RAND CONTROL Get info 5ug ($230)
Add to Cart
S790003 R03_PROM RAND CONTROL Get info 5ug ($230)
Add to Cart
S790004 R04_PROM RAND CONTROL Get info 5ug ($230)
Add to Cart
Empty promoter vector
Product # Name Description Coordinates Ordering  
S790005 EMPTY_PROM EMPTY VECTOR Get info FREE* Add to Cart

* One free empty vector construct (5ug) with any purchase

If you are interested in ordering more than 10ug of one or more control constructs, please contact our Sales Department for a custom quote.

Details

Empty Promoter VectorEmpty promoter vector: The empty promoter vector contains the RenSP luciferase gene without a promoter. This construct serves as a one measure of background signal in the experiment. This construct may also be used to create additional experimental constructs.

Housekeeping gene promoter vectors: Housekeeping control constructs contain promoters for common housekeeping genes driving the RenSP luciferase reporter. These constructs serve as positive transfection controls and may also serve as controls for comparing signals between conditions if they are known to be unresponsive to the test condition.

Random control vectors: Random control constructs contain 1 kb non-conserved, non-genic, and non-repetitive fragments from the human genome cloned upstream of the luciferase reporter. These vectors produce slightly different signals than the empty vectors and are considered optimal negative or background controls.

promoter chart Figure 1. Expected data and analysis of housekeeping and random promoter vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale. Control vector activity may vary depending on cell line, treatment condition, and protocol changes.