Science & Technology
Webinars
High-throughput functional screening for miRNA targets
Creating Pathway Profiling Panels: A Hypoxia Case Study
Understanding Promoter Technology
- Transfect constructs into cells.
- Apply stimulus. Transcription factors are activated and bind to promoter.
- Luciferase protein is made. Signal is read out on luminometer.
Understanding UTR Technology
- Transfect the SwitchGear 3’UTR construct into your cell line
- A constitutive promoter drives production of a hybrid luc2P-human 3’ UTR transcript
- Total luciferase output depends on the effect of the human 3’ UTR on the hybrid transcripts stability and/or translation efficiency
- Include a co-transfected synthetic miRNA or miRNA inhibitor to measure its impact on transcript stability and/or translation efficiency