Vector Information

LightSwitch™ Promoter Reporter Vector:

Prod ID	Type	Amount	Price
S790005	EMPTY_PROM	5ug	FREE*

Empty Promoter Vector Fragments may be cloned into the MCS to measure promoter activity. Standard promoter-reporter products include 1-3kb of human promoter sequence cloned into the MCS.

View the vector sequence and annotation file (txt)

Promoter Insert Sequencing Primers:

Forward: TCCATCAAAACAAAACGAAACAA

Reverse: AGTCGAGCACGTTCATCTGCTT

LightSwitch™ 3′UTR Reporter Vector:

Prod ID	Type	Amount	Price
S890005	EMPTY_3UTR	5ug	FREE*

Empty 3′UTR Reporter Vector Fragments cloned into the MCS downstream of the reporter gene will become part of a hybrid transcript that contains the luciferase coding sequence fused to the UTR sequence of interest. Standard 3′UTR-reporter products include 200bp-3kb human 3′UTR fragments cloned into the MCS.

View the vector sequence and annotation file (txt)

3′UTR Insert Sequencing Primers:

Forward: GGGAAGTACATCAAGAGCTTCGT

Reverse: CCCCCTGAACCTGAAACATAAA

LightSwitch™ 5′UTR Reporter Vector:

Prod ID	Type	Amount	Price
S690005	EMPTY_5UTR	5ug	FREE*

Empty 5′UTR Reporter Vector Fragments cloned into the MCS upstream of the reporter gene will become part of a hybrid transcript that contains the UTR sequence of interest fused to the luciferase coding sequence.

View the vector sequence and annotation file (txt)

5′UTR Insert Sequencing Primers:

Reverse: AGTCGAGCACGTTCATCTGCTT

LightSwitch™ Long-range Enhancer Reporter Vector:

Prod ID	Type	Amount	Price
S990005	EMPTY_LR	5ug	FREE*

Empty LR Reporter Vector Long-range transcriptional elements may be cloned into the MCS that is immediately upstream of a basal TK promoter to measure changes in transcriptional activity.

View the vector sequence and annotation file (txt)

LR Insert Sequencing Primers:

Forward: TCCATCAAAACAAAACGAAACAA

Reverse: AGTCGAGCACGTTCATCTGCTT

* One free empty vector construct (5ug) with any purchase