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Search for GoClonesProtocols Help

Protocols and Tech Notes

PROTOCOLS

LightSwitch™ GoClone® Reporter Constructs
Transfection Protocol for Adherent Cells
Transfection Protocol for K562 Suspension Cells
Transfection Protocol for 3′UTR Constructs and miRNA Mimic | Watch the video »
High-throughput Transfection Protocol

Reagents & Kits
LightSwitch™ Assay Reagent Protocol (LS010, 100 assays)
LightSwitch™ Assay Reagent Protocol (LS100, 1000 assays)
LightSwitch™ Dual Assay Kit Protocol (DA010, 100 assays)
FuGENE® HD Product Insert
Transfection Optimization Kit Protocol

HELP GUIDES

LightSwitch™ Luciferase Assay System Manual
Getting Started with Your Promoter Assays
Getting Started with Your 3′UTR Assays
3′UTR Assays Normalization Tutorial

TECHNICAL NOTES

Do I Need a Co-transfection Control?
High-throughput microRNA Target Screening: miR-122 Case Study (tech note)
High-throughput microRNA Target Screening: miR-122 Case Study (poster)
Using High-throughput Promoter Assays to Examine Regulatory Pathways
SwitchGear Methods for Gene Model Construction and Transcription Start Site Prediction

Promoters
Search Promoters
Promoter Controls
Synthetic Response Elements
Validated Pathway Assays
Pathway Reporter Cell Lines

3′UTRs and miRNAs
Search 3′UTRs
3′UTR Controls
Synthetic miRNA Targets
miRNA Mimics and Inhibitors

Empty Vector Suite
LightSwitch™ Assay Reagents
Transfection Reagents

ACTIVE MOTIF
Click here to visit the Active Motif website.

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