Heat Shock (HSF) Pathway Products

The SwitchGear Heat Shock Pathway Reporter Sets

The regulation of heat-shock genes is critical in cell stress response and the maintenance of cellular homeostasis. Heat-shock gene transcription is regulated by HSF (heat shock factor) transcription factors in response to various types of environmental stress such as heat, starvation, hypoxia, infection, and toxin exposure. Heat shock proteins (HSPs) are also well-characterized for their roles as chaperones for protein synthesis, assembly, secretion, and degradation. As specific HSPs have been implicated as central players in apoptosis, tumor survival, and immune response, understanding the regulation of the heat shock response is critical.

SwitchGear Genomics offers a comprehensive panel of cell-based reporter assays to allow you to accurately quantify transcriptional activation and repression across the heat shock pathway under various conditions. The SwitchGear Heat Shock promoter panels helps to reveal the coordinated action of genes responsible for adaptive responses to cell stress and the mechanism by which heat shock factors regulate normal development and disease conditions. In addition, SwitchGear offers UTR reporter assays that measure transcript stability, translational repression, miRNA function.


The SwitchGear collection of heat shock-relevant promoter reporters allows you to:

  • Understand the mechanisms by which heat shock-relevant genes are induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor level, our promoter reporter assays quantify the effects of transcription factor binding
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation or develop activity profiles across the heat shock signaling pathway for many compounds or conditions in parallel

SwitchGear also offers 3′UTR reporters for studying transcriptional stability, translational repression, and miRNA function. Read more about this technology.

Promoter Reporter Technology
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The Power of the LightSwitch Luciferase Assay System

  • Quantitative: Novel RenSP luciferase fusion technology allows you to measure promoter activity in response to heat shock (HSF) pathway activation with industry-leading sensitivity and dynamic range
  • Simple, quick, complete solution: Perform your experiments today through simple transfection of our luciferase reporter constructs using LightSwitch optimized reagents. No cloning, DNA preparation, or reagent optimization is needed. No need for co-transfection of normalizing controls.
  • Comprehensive and verified: Choose from SwitchGear’s complete set of sequence verified and transfection ready heat shock (HSF) targets.
  • Functionally insightful: Learn about the actual effects of transcription factor binding to verify computational predictions and supplement microarray or sequencing data.
  • Cost-effective: Profile promoter activation efficiently across the full heat shock (HSF) pathway under a multitude of conditions in parallel.

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Getting started with SwitchGear promoter constructs