NF-kB Pathway Products

The SwitchGear NF-kB Pathway Sets

NF-kB is a ubiquitous transcription factor that regulates the genes that mediate cell cycle, apoptosis, intercellular communication, immune response and development, memory formation, and tumorigenesis. Various signals such as intracellular stress, cytokines, UV irradiation, free radicals, and infection induce gene expression through the NF-kB family. NF-kB family members — NF-kB1 (p50), NF-kB2 (p52), RelA (p65), RelB, and c-Rel share a highly conserved domain responsible for DNA binding to NF-kB-elements. Improper regulation of NF-κB has been associated with various cancers, autoimmune diseases, inflammatory disorders, and viral infection. Understanding the NF-kb pathway may aid in identifying preventive and therapeutic measures for diseases, such as chronic inflammation and cancer.

SwitchGear Genomics offers a comprehensive panel of transfection-ready, cell-based promoter reporter assays to allow you to accurately quantify transcriptional activation and repression in response to NF-kB pathway activation. In addition, SwitchGear offers UTR reporter assays that measure transcript stability, translational repression, miRNA function.

Applications

The SwitchGear collection of NF-kB -relevant promoter reporters allows you to:

  • Understand the mechanisms by which NF-kB regulated genes are induced or repressed
  • Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor level, our promoter reporter assays quantify the effects of transcription factor binding
  • Confirm data from ChIP-chip or ChIP-sequencing experiments
  • Measure the effect of sequence variants and mutagenesis on promoter function
  • Screen for promoter activation or develop activity profiles across the NF-kB signaling pathway for many compounds or conditions in parallel

SwitchGear also offers 3′UTR reporters for studying transcriptional stability, translational repression, and miRNA function. Read more about this technology.

Promoter Reporter Technology
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The Power of the LightSwitch Luciferase Assay System

  • Quantitative: Novel RenSP luciferase fusion technology allows you to measure promoter activity in response to NF-kB pathway activation with industry-leading sensitivity and dynamic range
  • Simple, quick, complete solution: Perform your experiments today through simple transfection of our luciferase reporter constructs using LightSwitch optimized reagents. No cloning, DNA preparation, or reagent optimization is needed. No need for co-transfection of normalizing controls.
  • Comprehensive and verified: Choose from SwitchGear’s complete set of sequence verified and transfection ready NF-kB targets.
  • Functionally insightful: Learn about the actual effects of transcription factor binding to verify computational predictions and supplement microarray or sequencing data.
  • Cost-effective: Profile promoter activation efficiently across the full NF-kB pathway under a multitude of conditions in parallel.

Additional links

Getting started with SwitchGear promoter constructs

NF-kB in disease: Application highlight

Inflammatory disease: NF-κB is a key player in inflammatory disorders such as arthritis, sepsis, and asthma, and inflammatory bowel disease. Many small molecules with anti-inflammatory effects have also been shown to inhibit NF-κB. Understanding the activation and repression of genes in the NF-kb pathway provides greater value in developing potential therapeutics targeting NF-kb in inflammatory disease.

Oncology: As NF-κB is a regulator of genes that control cell proliferation, survival, and apoptosis, many tumors have constitutively active NF-kb. Blocking NF-κB activity has been shown to cease tumor cell proliferation or induce apoptosis. Accordingly, NF-κB is the subject of research as a target for anti-cancer therapy.