Cholesterol Biosynthesis (SREBP) Pathway Products
SwitchGear Cholesterol Biosynthesis (SREBP) Pathway Screening Sets
The sterol regulatory element binding proteins (SREBPs) are transcription factors that regulate lipogenesis. The SREBP isoforms, SREBP-1a, SREBP-1c, and SREBP-2, specifically control the expression of genes involved in cholesterol, fatty acid, triglyceride, and phospholipid synthesis. SREBP-1c has also been implicated in glucose uptake regulation. Dysregulation of SREBP and the SREBP-regulated pathways is clinically relevant in disorders such as diabetes mellitus, insulin resistance, non-alcoholic fatty liver disease (NAFLD), and obesity. Understanding the mechanisms of how SREBPs are coordinated with other physiological pathways gives further insight into the roles of SREBP in normal development and can lead to successful strategies for targeting SREBP pathological research and drug therapies.
SwitchGear Genomics offers a comprehensive panel of cell-based reporter assays that allow you to accurately quantify transcriptional activation and repression in response to cholesterol biosynthesis (SREBP) pathway activation. Our promoter assays includes an empirically-validated collection of promoters of genes that are targets of SREBP. The SwitchGear SREBP promoter reporters help to reveal the coordinated action of genes responsible in the SREBP pathway and the mechanism by which these genes regulate normal development and disease conditions. In addition, SwitchGear offers UTR reporter assays that measure transcript stability, translational repression, miRNA function.
Applications
The SwitchGear collection of SREBP-relevant promoter reporters allows you to:
- Understand the mechanisms by which SREBP regulated genes are induced or repressed
- Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor level, our promoter reporter assays quantify the effects of transcription factor binding
- Confirm data from ChIP-chip or ChIP-sequencing experiments
- Measure the effect of sequence variants and mutagenesis on promoter function
- Screen for promoter activation or develop activity profiles across the SREBP signaling pathway for many compounds or conditions in parallel
SwitchGear also offers 3′UTR reporters for studying transcriptional stability, translational repression, and miRNA function. Read more about this technology.
Promoter Reporter Technology
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The Power of the LightSwitch Luciferase Assay System
- Quantitative: Novel RenSP luciferase fusion technology allows you to measure promoter activity in response to SREBP pathway activation with industry-leading sensitivity and dynamic range
- Simple, quick, complete solution: Perform your experiments today through simple transfection of our luciferase reporter constructs using LightSwitch optimized reagents. No cloning, DNA preparation, or reagent optimization is needed. No need for co-transfection of normalizing controls.
- Comprehensive and verified: Choose from SwitchGear’s complete set of sequence verified and transfection ready SREBP targets.
- Functionally insightful: Learn about the actual effects of transcription factor binding to verify computational predictions and supplement microarray or sequencing data.
- Cost-effective: Profile promoter activation efficiently across the full SREBP pathway under a multitude of conditions in parallel.