Cholesterol Biosynthesis (SREBP) Pathway Products


SwitchGear Genomics offers a panel of 88 transfection-ready reporter constructs to allow you to measure cholesterol biosynthesis (SREBP) pathway activation. The SwitchGear cholesterol biosynthesis (SREBP) promoter targets were picked from our genome-wide promoter collection based on sequence motif analysis, expression studies, and transcription factor binding data. We conducted experiments to generate a profile across the set of constructs with and without SREBP activation. These data are summarized in the heatmap below. SwitchGear offers the complete pathway profiling panel of constructs in a plate format that allows you to characterize the effects of many conditions across the entire SREBP pathway. We also offer a smaller subset of key responder constructs that show a strong induction response and can be used as biomarkers in screening applications.

Cholesterol Biosynthesis (SREBP) Biomarker Set - 8 promoters, 2 controls

srebp_biomarkersThe selected constructs shown in the graph are the 8 of the highest responding promoters under our chosen test conditions*. The chart summarizes the induction ratios for each construct in HepG2 human hepatocarcinoma cells that were transfected with promoter-reporter constructs and treated with 1uM lovastatin or vehicle only for 24 hours in reduced lipid media. The Y-axis shows the treated/untreated signal ratio on a log scale and error bars represent one standard deviation. Please see the heatmap below for relative promoter activities in an untreated setting.

Cholesterol Biosynthesis (SREBP) Profiling Plate - 88 promoters, 8 controls

srebpClick on the image for a larger view and then utilize the zoom tool in your browser window.

The selected constructs (as shown in the heatmap) represent the SwitchGear Cholesterol Biosynthesis (SREBP) Profiling Plate. The heatmap summarizes the untreated activity and induction response for each construct in the pathway profiling plate using one common induction strategy (see above)*. On the right side of the figure, the colors represent log2 transformed ratios of treated/untreated activity according to the color scale shown at the base of the heatmap. Black indicates no change, and intensity of red or blue indicates levels of induction or repression, respectively. On the left side, the intensity of yellow parallels the activity of the promoter in the unstimulated or untreated condition. All untreated signals are relative to the highest and lowest values within the individual experiment. (“LOV”= HepG2 cells treated with 1 uM lovastatin for 24 hours; “SYNTH”= HepG2 cells treated with 5 ug/ml synthechol for 24 hours; “U18”= HepG2 cells treated with 1 uM U18666A for 24 hours).

View plate map (xls)

*Experimental results and absolute values of luciferase units may vary depending on cell line, treatment, and protocols. The data should be used as a guide for selecting candidate promoters and panels for your particular experimental design.