Serum Response Factor (SRF) Pathway Products

Introduction

SwitchGear Genomics offers transfection-ready reporter constructs to allow you to measure serum response factor (SRF) pathway activation. The SwitchGear SRF promoter targets were picked from our genome-wide promoter collection based on sequence motif analysis, expression studies, and transcription factor binding data. We conducted experiments to generate a profile across the set of constructs with and without SRF activation and offer a set of key responder constructs that show a strong induction response that can be used as biomarkers in screening applications.

Serum Response Factor (SRF) Biomarker Set – 4 promoters, 2 controls

The selected constructs shown in the graph are the 4 of the highest responding promoters under our chosen test conditions*. The chart summarizes the induction ratios for each construct in serum-starved HT1080 human fibrosarcoma cells that were transfected with promoter-reporter constructs and were either left in serum starvation media or exposed to media containing 20% FBS for 8 hours. The Y-axis shows the treated/untreated signal ratio on a log scale and error bars represent one standard deviation.