Protocols and Tech Notes

PROTOCOLS

LightSwitch™ GoClone^® Reporter Constructs
Transfection Protocol for Adherent Cells
Transfection Protocol for K562 Suspension Cells
Transfection Protocol for 3′UTR Constructs and miRNA Mimic | Watch the video »
High-throughput Transfection Protocol

Reagents & Kits
LightSwitch™ Assay Reagent Protocol (LS010, 100 assays)
LightSwitch™ Assay Reagent Protocol (LS100, 1000 assays)
LightSwitch™ Dual Assay Kit Protocol (DA010, 100 assays)
FuGENE^® HD Product Insert
Transfection Optimization Kit Protocol

HELP GUIDES

LightSwitch™ Luciferase Assay System Manual
Getting Started with Your Promoter Assays
Getting Started with Your 3′UTR Assays
3′UTR Assays Normalization Tutorial

TECHNICAL NOTES

Do I Need a Co-transfection Control?
High-throughput microRNA Target Screening: miR-122 Case Study (tech note)
High-throughput microRNA Target Screening: miR-122 Case Study (poster)
Using High-throughput Promoter Assays to Examine Regulatory Pathways
SwitchGear Methods for Gene Model Construction and Transcription Start Site Prediction