The LightSwitch™ Promoter Reporter GoClone® Collection
Our comprehensive collection contains more than 18,000 endogenous human promoter reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity.
The LightSwitch™ Promoter Reporter GoClone® collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 18,000 human promoters and highly optimized LightSwitch reagents. Measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminates the need for co-transfection in most studies.
Applications
Measure promoter activity to:
- Understand the mechanism by which a gene is induced or repressed
- Characterize the functional consequences of transcription factor binding
- Screen full regulatory networks with SwitchGear’s Pathway Screening Panels
- Measure the effect of sequence variants on promoter function (see our Sequence Variant Assay Services)
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Using high-throughput promoter reporter assays to examine regulatory pathways |
LightSwitch™ Promoter Reporter GoClone® Technology Click on the image below for a larger view.
The Power of the LightSwitch™ Luciferase Assay System
- Quantitative: Novel RenSP luciferase technology allows you to measure promoter activity with industry-leading sensitivity and dynamic range.
- Simple, quick, complete solution: Perform your experiments today through simple transfection of our luciferase reporter constructs using LightSwitch optimized reagents. No cloning, DNA preparation, or reagent optimization is needed. No need for internal transfection control for most studies.
- Comprehensive and verified: Choose from SwitchGear’s genome-wide sequence-verified and transfection-ready promoter targets.
- Functionally insightful: Learn about the actual effects of transcription factor binding to verify computational predictions and supplement microarray or sequencing data.
- Cost-effective: Screen for promoter activation and repression under a multitude of conditions efficiently.