miRNA Target Validation

Validate the targets of your favorite miRNAs

Understanding UTR

  1. Transfect the GoClone 3′UTR construct into your cell line
  2. A constitutive promoter drives production of a hybrid RenSP-human 3′UTR transcript
  3. Total luciferase output depends on the effect of the human 3′UTR on the hybrid transcripts stability and/or translation efficiency
  4. Include a co-transfected synthetic miRNA or miRNA inhibitor to measure its impact on transcript stability and/or translation efficiency
  5. Use optimized LightSwitch Luciferase Reagents to obtain maximun sensitivity and dynamic range