UTR Reporter GoClone Collection Control Vectors

SwitchGear offers a number of control constructs to optimize experimental conditions and to normalize for non-specific events that may cause signal variation. We recommend including at least one control of each type listed below to minimize the possibility of experimental conditions interacting with a control.

Empty 3’UTR vector: The empty 3’UTR vector contains only the luciferase gene (Luc2P) and its constitutive promoter with the multiple cloning site removed. This construct may serve as a positive control for the transfection.

Housekeeping gene 3’UTR vectors: Housekeeping control constructs contain the 3’UTRs for common housekeeping genes cloned downstream of the luciferase reporter. See SwitchDB, our online catalog, for details about these vectors.

Random 3’UTR control vectors: Random control constructs contain non-conserved, non-genic, and non-repetitive human genomic fragments.

See SwitchDB, our online catalog, for details about these vectors.

	Figure 1. Example data for SwitchGear 3’UTR control vectors. Here 50ng of each vector was transfected with FuGene6 (Roche) into HT1080 cells. Absolute and relative activity will vary depending on cell line, experimental conditions, and transfection reagents.
Values shown in log scale. Relative activities will differ depending on cell type and conditions.