Products
Begin your experiments today - order SwitchGear’s Assay-Ready Constructs.
SwitchGear provides sequence-verified and assay-ready promoter and UTR reporter constructs across the entire human genome. Constructs from our collection are delivered as purified, transfection-ready plasmid DNAs for use with Promega’s luciferase assays. Customers can order individual constructs, complete experimentally-validated pathway sets, or customized sets. We also offer luciferase reagents optimized to work with SwitchGear products.
Assay-Ready Constructs
Sequence-verified and transfection-ready luciferase reporter constructs for over 17,000 human promoters. Measure the functional response of promoters to stimuli such as transcription factor binding in nearly any cell-based system. Search our online catalog, SwitchDB.
Sequence-verified and transfection-ready luciferase reporter constructs for over 10,000 human 3’UTRs. Measure RNA stability, translational efficiency, and the functional impact of miRNAs on any gene. Search our online catalog, SwitchDB.
These sets of promoter targets from our genome-wide promoter reporter collection have been experimentally validated with pathway-specific inductions to create pathway activity profiles. We offer validated pathway biomarker sets for CREB, NF-kB, p53, nuclear receptors, hypoxia response (HIF1a), cholesterol biosynthesis (SREBP), interferon response (STAT1), and more. Use these panels and our high-throughput transfection protocol for a detailed analysis of regulatory pathways in living cells.
SwitchGear provides both promoter and UTR control constructs to optimize and monitor the transfection efficiency of your experimental system. In addition, SwitchGear offers various controls to normalize for the non-specific effects of your experiments.
SwitchGear is committed to providing a full solution for your reporter assay needs and now offers the option to add Promega’s luciferase reagents to any order. Simply log into our online catalog, SwitchDB, and choose everything you need to get started today – transfection-ready experimental constructs, controls, and reagents. We offer the following reagents that have been optimized to work with SwitchGear products (reagent offering available to United States customers only):